IonGate Biosciences

Document Library

Overview

Assays
Mitochondrial Transport Investigated with SURFE²R Technology (460.1 kB)
Datasheet
Applications on SURFE²R (282.8 kB)
Datasheet

Assay Development

Customer-specific assay development for electrogenic membrane proteins

Take the competitive lead with IonGate Customized Assay Development Services (671.5 kB)

Brochure

Case Studies
SURFE²R Technology for pharmaceutical studies
of the influenza A-M2 channel (674.0 kB)
Case Study

Application Notes

The SURFE²R Workstation 500: An assay performance evaluation

The SURFE²R Workstation 500: An assay performance evaluation

SURFE²R™ Technology is an established tool for the investigation of transport protein activity. It has recently been transferred from a single sensor-based system to a 96 sensor platform called SURFE²R Workstation 500. This application note focuses on the assessment of the assay performance of the SURFE²R Workstation 500 by means of the Z’-factor determined for a human sodium/calcium exchanger (NCX1) assay.

The SURFE²R Workstation 500: An assay performance evaluation (1.9 MB)
Application Note
The use of the SURFE²R Technology for rapid characterisation of the affinity and mechanism of new proton pump inhibitors.

The use of the SURFE²R Technology for rapid characterisation of the affinity and mechanism of new proton pump inhibitors.

Although proton pump inhibitors are a well-known class of drugs, there is still a to date unmet need for new compounds that inhibit HK-ATPase. The desired mechanism of action of these compounds is determined by its reversibility, thereby enabling a short-term inhibition for the treatment of symptoms found in GERD. Using conventional technologies, the evaluation of the mechanism is cumbersome and in some cases not able to detect all reversible compounds...
The use of the SURFE²R Technology for rapid characterisation of the affinity and mechanism of new proton pump inhibitors (255.6 kB)
Application Note
Small Compartments with Great Impact

Small Compartments with Great Impact

Discover the Mitochondrion

Mitochondria are exceptional little “creatures”: believed to be originally derived endosymbionts they became the powerhouses of our cells. They are a bit alien and for that reason it has not been possible to draw all their secrets so far. Mitochondria are important in a wide range of diseases such as cancer, diabetes, obesity or age-related neurodegenerative diseases. IonGate now has developed a method to analyse transport proteins in native mitochondrial membranes. To meet especially the needs of academic research seeking to understand and decipher the impact of mitochondria in various diseases, the new SURFE²R Workstation 5 will be launched early in 2009.

Small Compartments with Great Impact (278.5 kB)
Application Note

Scientific Publications

Scientific publications related to SSM-based electrophysiology and SURFE²R

Scientific publications related to SSM-based electrophysiology and SURFE²R

Scientific publications related to SSM-based electrophysiology and SURF2R

(494.6 kB)
Surfing on the Calcium Waves to Understand Heart Rhythm

Surfing on the Calcium Waves to Understand Heart Rhythm

Surfing on the Calcium Waves to Understand Heart Rhythm (211.4 kB)
Publication: SCREENING 03/2008, pp 10–11, GIT VERLAG GmbH & Co. KG, Darmstadt
Transport Proteins: The Final Frontier?

Transport Proteins: The Final Frontier?

From Clamping to Surfing in No Time

Transport Proteins: The Final Frontier? (512.1 kB)
Publication: Screening 02/2008, S. 36-37, GIT VERLAG GmbH & Co. KG, Darmstadt
Advanced Transporter-based Drug Discovery

Advanced Transporter-based Drug Discovery

Research and Innovation in Cell-free Assay Development
Advanced Transporter-based Drug Discovery (330.3 kB)
Publication: Screening 01/2008, S. 38, GIT VERLAG GmbH & Co. KG, Darmstadt
Opening the Gates to a Novel World

Opening the Gates to a Novel World

Or Why a Peek through the Keyhole Is Just So Irresistible
Opening the Gates to a Novel World (1.3 MB)
Publication: BIOforum, Volume 11, October 2007
A fast key for slow gatekeepers

A fast key for slow gatekeepers

Wolfgang Lerch, Managing Director at Iongate Biosciences, reports on an exciting technological development, in use for just over a year, which accesses the transporters using cell-free electrophysiology.
A fast key for slow gatekeepers (735.2 kB)
Publication: WorldPharmaceuticalFrontiers

Further reading

  • P. Schulz, J. J. Garcia-Celma, K. Fendler (2008)
    SSM-based electrophysiology. Methods 46, 97-103.
  • S. Geibel, N. Flores-Herr, T. Licher and Vollert H. (2006)
    Establishment of Cell-Free Electrophysiology for ion transporters: application for pharmacological profiling.
    Journal of Biomeolecular Screening, 11(3), 262-268.
  • B. Kelety, K. Diekert, J. Tobien, N. Watzke, W. Dörner, P. Obrdlik and K. Fendler (2006)
    Transporter assays using solid supported membranes: a novel screening platform for drug discovery.
    Assay and Drug Development Technologies. 4(5), 575-582.

Posters

Cell-free electrophysiology of the Na+/Pi cotransporter NaPi-llb in mammalian cells

Natalie Watzke1, Kerstin Diekert1, Ingo Janausch1, Jürg Biber2, Ian C. Forster2 and Petr Obrdlik1

1IonGate Biosciences GmbH, Industrial Park Hoechst, Frankfurt a.M., Germany

2Inst. of Physiology and Centre of Integrative Human Physiology, University of Zurich, Switzerland

Cell-free electrophysiology of the Na+/Pi cotransporter NaPi-llb in mammalian cells (2.0 MB)
Poster Bacterial Transporter

The SURFE²R Technology as a tool for measuring electrophysiological transport activity of different classes of transporters

Ingo G. Janausch1, Petr Obrdlik2, Robin Krause3, Joachim Englert2, Klaus Fendler4, Dietmar Weitz5, Hannelore Daniel5 and Bela Kelety2

IonGate Biosciences, Frankfurt am Main, Germany:
1 Molecular and Cell Biology 2 Assay Development 3 Development and Production
4 Biophysical Chemistry, Max Planck Institute of Biophysics, Frankfurt am Main, Germany
5 Lehrstuhl für Ernährungsphysiologie, TU München, Freising, Germany

The SURFE²R Technology as a tool for measuring electrophysiological transport activity of different classes of transporters (568.4 kB)
Poster
Cell-free electrophysiology of the viral H+ channel M2.

Cell-free electrophysiology of the viral H+ channel M2.

Petr Obrdlik1, Catrin Steensen1, Victoria Balannik2, Joshua Rausch2,
Lawrence Pinto2 & Béla Kelety1

1IonGate Biosciences, Frankfurt am Main, Germany

2Dep. Neurobiology & Physiology, Northwestern University, Evanston, IL, USA

Cell-free electrophysiology of the viral H+ channel M2 (2.9 MB)
Poster
A robust electrical method to investigate regulation and expression of transport proteins on a functional level in whole cells

A robust electrical method to investigate regulation and expression of transport proteins on a functional level in whole cells

Ingo G. Janausch, Inga Barth, Kerstin Diekert, Sabine Molitor, Nicole Wiegand, Joachim Englert, Petr Obrdlik & Béla Kelety
A robust electrical method to investigate regulation and expression of transport proteins on a functional level in whole cells (1.8 MB)
Poster
A novel assay technique for drug discovery in neuronal glutamate uptake

A novel assay technique for drug discovery in neuronal glutamate uptake

Robin Krausea,b, Natalie Watzkeb, Klaus Fendlera, Wolfgang Dörnerb, Béla Keletyb

a Max-Planck-Institut für Biophysik, Max-von-Laue-Str. 3, D-60438 Frankfurt am Main
b IonGate Biosciences GmbH, Industriepark Höchst D528, D-65926 Frankfurt am Main

A novel assay technique for drug discovery in neuronal glutamate uptake (555.1 kB)
Poster
Electrophysiology of native mitochondrial transporters, respiratory chain complexes, and oxidative phosphorylation

Electrophysiology of native mitochondrial transporters, respiratory chain complexes, and oxidative phosphorylation

Natalie Watzke, Kerstin Diekert, Petr Obrdlik,

IonGate Biosciences, Industriepark Höchst, D528, Frankfurt am Main, Germany

Electrophysiology of native mitochondrial transporters, respiratory chain complexes, and oxidative phosphorylation (1.6 MB)
Poster
Cell-free electrophysiology of glucose transporter rbSGLT1

Cell-free electrophysiology of glucose transporter rbSGLT1

Detection of proteins in reverse and forward transport mode

Petr Obrdlik, Catrin Steensen, Natalie Watzke, Inga Bick, Martina Schreiter, Maarten Ruitenberg & Béla Kelety

Cell-free electrophysiology of glucose transporter rbSGLT1 (1.7 MB)
Poster
The SURFE²R One System for the Investigation of Sodium-coupled Transporters

The SURFE²R One System for the Investigation of Sodium-coupled Transporters

Natalie Watzke, Petr Obrdlik, Atia Arif, Ulrich Pehl, Kerstin Diekert, Renate Gauß, Wolfgang Dörner, Marçal Pastor-Anglada*, Béla Kelety

IonGate Biosciences GmbH, Industriepark Höchst D528, D-65926 Frankfurt/Main, Germany;
*Biochemistry and Molecular Biology, University of Barcelona, Barcelona, Spain

The SURFE²R One System for the Investigation of Sodium-coupled Transporters (1.1 MB)
Poster
Direct electrical measurements of V-ATPase and Na+/K+-ATPase in native vesicles and plasma membranes

Direct electrical measurements of V-ATPase and Na+/K+-ATPase in native vesicles and plasma membranes

Petr Obrdlik, Christine Keipert, Natalie Watzke, Kerstin Diekert, Ulrich Pehl, Inga Bick, Catrin Steensen & Béla Kelety
Direct electrical measurements of V-ATPase and Na+/K+-ATPase in native vesicles and plasma membranes (1.1 MB)
Poster

Products

SURFE²R Workstation 5

SURFE²R Workstation 5

System Factsheet (198.3 kB)
SURFE²R Workstation 5
Technical Specifications (142.7 kB)
SURFE²R Workstation 5
SURFE²R Workstation 50

SURFE²R Workstation 50

System Factsheet (504.2 kB)
SURFE²R Workstation 50
Technical Specifications (143.1 kB)

SURFE²R Workstation 50
SURFE²R Workstation 500

SURFE²R Workstation 500

System Factsheet (806.0 kB)
SURFE²R Workstation 500
Technical Specifications (141.2 kB)
SURFE²R Workstation 500
SURFE²R Workstation 5000

SURFE²R Workstation 5000

System Factsheet (792.8 kB)
SURFE²R Workstation 5000
Technical Specifications (141.5 kB)
SURFE²R Workstation 5000